Composite

Part:BBa_K1124102

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

clock device (plux/tet) (+luxI, -tetR, -aiiA, --sRNA, +amilGFP)(positive feedback only)

The construct for Multicellular Analog Clock. This BBa_K1124102 contains only positive feedback. You can check the positive feedback loop of the clock "gear". 

 LuxI and LuxR forms the positive feedback loop of AHL production.
 mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).
 amilGFP is the yellow reporter protein expressed constitutively as an internal standard.

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1666
    Illegal NheI site found at 1689
    Illegal NheI site found at 2653
    Illegal NheI site found at 2676
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None